Application
Western blot
Sample
Xenopus laevis Cell lysate - other (Egg)
Loading amount
25 µg
Specification
Egg
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s) · Diluent: TBS-Tween0,1% Milk 5%
Secondary antibody
Name
Non-Abcam antibody was used: ECL Donkey anti-rabbit IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL
Exposure
1 hour(s), 0 minute(s) and 0 second(s)
Bands
Specific: 93 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Aug 03 2012