参阅全部 MATH2/NEUROD6 一抗
经测试应用适用于: WB, ICC/IFmore details
种属反应性与反应: Mouse, Rat, Human
预测可用于: Cow, Monkey
- 购买相配的WB阳性对照Recombinant Human MATH2/NEUROD6 protein
- This antibody gave a positive signal in the following tissue lysates: Human brain; Mouse brain; Rat brain, as well as the following whole cell lysates: PC12; U87mg; U373 MG Nuclear.
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
纯度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab85824 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 38 kDa).|
|ICC/IF||Use a concentration of 5 µg/ml.|
功能Activates E box-dependent transcription in collaboration with TCF3/E47. May be a trans-acting factor involved in the development and maintenance of the mammalian nervous system. Transactivates the promoter of its own gene.
序列相似性Contains 1 basic helix-loop-helix (bHLH) domain.
- Information by UniProt
- Atoh 2 antibody
- Atoh2 antibody
- Atonal homolog 2 antibody
All lanes : Anti-MATH2/NEUROD6 antibody (ab85824) at 1 µg/ml
Lane 1 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 2 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate
Lane 3 : Brain (Mouse) Tissue Lysate
Lane 4 : Brain (Rat) Tissue Lysate
Lane 5 : Human brain tissue lysate - total protein (ab29466)
Lane 6 : U373 MG (Human glioblastoma-astrocytoma, epithelial-like cell line) Nuclear Lysate (ab14902)
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 38 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa, 120 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 16 minutes
ICC/IF image of ab85824 stained PC12 cells. The cells were 100% Methanol fixed (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab85824 at 5ug/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti- Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
- Sullivan CS et al. Perineuronal Net Protein Neurocan Inhibits NCAM/EphA3 Repellent Signaling in GABAergic Interneurons. Sci Rep 8:6143 (2018). Read more (PubMed: 29670169) »
- Tachibana N et al. Pten Regulates Retinal Amacrine Cell Number by Modulating Akt, Tgfß, and Erk Signaling. J Neurosci 36:9454-71 (2016). Read more (PubMed: 27605619) »