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Pre-cut membranes (10 sheets, 7 x 9 cm), compatible with gels 10 x 10 cm and smaller. Note: PVDF membranes must be activated prior to use. Membrane activation protocol: 1. Pre-wet the membrane in methanol for a few moments until the membrane is completely wet. 2. Transfer the wet membrane from methanol to purified water and incubate on a rocker or shaker for at least 5 minutes. Make sure that the membrane is fully immersed in water and does not float on the water’s surface. 3. Transfer the membrane from water to transfer buffer, and incubate for 5 minutes.
Our Abpromise guarantee covers the use of ab133411 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"