Application
Western blot
Sample
Mouse Cell lysate - whole cell (Bone Marrow-Derived Macrophages)
Gel Running Conditions
Reduced Denaturing (10% polyacrylamide gel)
Loading amount
160000 cells
Treatment
20 ng/mL mouse IL-4 for 24 hours
Specification
Bone Marrow-Derived Macrophages
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 5% non-fat milk/PBST
Dilution
1/1000
Secondary antibody
Dilution
1/3000
Name
Non-Abcam antibody was used: Goat anti-rabbit IgG HRP-linked antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Amersham ECL Select
Negative control
50 ng/mL mouse IFN-? +100 ng/mL LPS for 24 hours
Exposure
15 second(s)
Bands
Specific: 40, 42 kDa Non-specific: 30 kDa
Positive control
20 ng/mL mouse IL-4 for 24 hours
Additional data
Additional Notes
Type of membrane used: nitrocellulose
Lysis buffer used: 1 × SDS sample buffer (50 mM Tris-HCl, 1% SDS, 5% glycerol, 0.01% bromophenol blue, pH 6.8)
PubMed ID
27588757: View
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
祐介 安藤
Verified customer
提交于 Nov 16 2016