Application
Western blot
Sample
Human Cell lysate - whole cell (Peripheral blood mononuclear cells)
Gel Running Conditions
Non-reduced Denaturing (12% Tris-HEPES-SDS)
Loading amount
20 µg
Specification
Peripheral blood mononuclear cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: 5% milk blocking solution
Dilution
1/300
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Sant Cruz sc-2030 Goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
SuperSignal West Pico Chemiluminescent
Exposure
5 second(s)
Bands
Specific: 69 kDa
Additional data
Additional Notes
Cells lysed in laemmli buffer with phosphotase and protease inhibitors. Nitrocellulose membrane used.
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提交于 Mar 31 2009