antigen sequence: LRCIEFPVPK DCYPEGAAYP QDELAMSKFN QQKYDEIAEE LRAVLLRADR EDIQVSTPLF GSFDPDIQET SNELGAFLLQ AFKTAL, corresponding to C terminal amino acids 418-503 of Human LRRC14B protein (UniProt ID: AGNHZ5).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Belongs to the PRAME family.
Contains 5 LRR (leucine-rich) repeats.
Information by UniProt
Leucine-rich repeat-containing protein 14B antibody
Immunocytochemistry/ Immunofluorescence - Anti-LRRC14B antibody (ab122435)
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleoli. Recommended concentration of ab122435 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRRC14B antibody (ab122435)
ab122435, at a 1/50 dilution, staining LRRC14B in paraffin embedded Human bronchus tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
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