LDH Assay试剂盒 (Cytotoxicity) (ab65391)
Key features and details
- Assay type: Enzyme activity
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Adherent cells, Suspension cells
概述
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产品名称
LDH Assay试剂盒 (Cytotoxicity)
参阅全部 Lactate Dehydrogenase 试剂盒 -
样品类型
Adherent cells, Suspension cells -
检测类型
Enzyme activity -
检测时间
1h 00m -
产品概述
For a more sensitive LDH Assay Kit to use as a cytotoxicity assay, we recommend ab65393.
LDH Assay Kit (Cytotoxicity) ab65391 provides a fast and simple method for quantitating cytotoxicity based on the measurement of activity of lactate dehydrogenase (LDH) released from damaged cells.
The assay takes ~0.5-1 hr. LDH activity can be determined by a coupled enzymatic reaction: LDH oxidizes lactate to pyruvate which then reacts with tetrazolium salt INT to form formazan. The increase in the amount of formazan produced in culture supernatant directly correlates to the increase in the number of lysed cells. The formazan dye is water-soluble and can be detected by spectrophotometer at 500 nm.
This kit was previously called LDH-Cytotoxicity Assay Kit I.
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说明
This product is manufactured by BioVision, an Abcam company and was previously called K311 LDH-Cytotoxicity Colorimetric Assay Kit. K311-400 is the same size as the 400 test size of ab65391.
Cell death or cytotoxicity is classically evaluated by the quantification of plasma membrane damage. Unlike many other cytoplasmic enzymes which exist in many cells either in low amount (e.g., alkaline and acid phosphatase) or unstable, LDH is a stable cytoplasmic enzyme present in all cells and rapidly released into the cell culture supernatant upon damage of the plasma membrane.
If you would like to use a fluorometric reading, please refer to LDH-Cytotoxicity Assay Kit (Fluorometric) (ab197004).
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平台
Microplate reader
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 400 tests 8000 tests LDH Catalyst Reagent 1 vial 20 vials Dye Solution I 1 x 45ml 20 x 45ml -
研究领域
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通路
Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1. -
疾病相关
Defects in LDHA are the cause of glycogen storage disease type 11 (GSD11) [MIM:612933]. A metabolic disorder that results in exertional myoglobinuria, pain, cramps and easy fatigue. -
序列相似性
Belongs to the LDH/MDH superfamily. LDH family. -
翻译后修饰
ISGylated. -
细胞定位
Cytoplasm. - Information by UniProt
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别名
- Cell proliferation-inducing gene 19 protein
- GSD11
- L lactate dehydrogenase B chain
see all
相关产品
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Assay kits
图片
数据表及文件
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SDS download
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Datasheet download
文献 (35)
ab65391 被引用在 35 文献中.
- Li Y et al. Scutellaria barbata Inhibits Hepatocellular Carcinoma Tumorigenicity by Inducing Ferroptosis of Hepatocellular Carcinoma Cells. Front Oncol 12:693395 (2022). PubMed: 35321425
- Borkar NA et al. Kisspeptins inhibit human airway smooth muscle proliferation. JCI Insight 7:N/A (2022). PubMed: 35420998
- Tolardo V et al. In Vitro High-Throughput Toxicological Assessment of Nanoplastics. Nanomaterials (Basel) 12:N/A (2022). PubMed: 35745286
- Hyldbakk A et al. Identification of novel cyanoacrylate monomers for use in nanoparticle drug delivery systems prepared by miniemulsion polymerisation - A multistep screening approach. Int J Pharm X 4:100124 (2022). PubMed: 35898812
- Kong X et al. Transcranial Direct-Current Stimulation Regulates MCT1-PPA-PTEN-LONP1 Signaling to Confer Neuroprotection After Rat Cerebral Ischemia-Reperfusion Injury. Mol Neurobiol 59:7423-7438 (2022). PubMed: 36190692