Participates in the connection between ECM adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Recruits migfilin (FBLP1) protein to cell-ECM focal adhesion sites.
Ubiquitous. Found in numerous tumor tissues.
Belongs to the kindlin family. Contains 1 FERM domain. Contains 1 PH domain.
The FERM domain is not correctly detected by PROSITE or Pfam techniques because it contains the insertion of a PH domain.
Kindlin 2 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Kindlin 2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation. Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab74030. Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697). Band: 78kDa; Kindlin 2
Western blot - Anti-Kindlin 2 antibody (ab74030)This image is courtesy of an abreview submitted by Ana Kasirer-Friede,UCSD , United States
The kindlin 2 antibody (ab74030) was tested in lysate obtained from CHO cells either expressing endogenous levels of kindlin 2 (lane1)and transfected CHO cells in which flag-tagged kindlin2 was overexpressed (lane 2). A specific band was at the expected molecular weight of approximately 72 kDa (+/- Flag-tag). ß-actin was used to show loading in the two lanes.
Immunocytochemistry/ Immunofluorescence - Anti-Kindlin 2 antibody (ab74030)Image courtesy of an anonymous Abreview.
ab74030 staining Kindlin 2 in HeLa cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldehyde, permeabilized using Triton, then incubated with ab74030 at a 1/100 dilution for 1 hour at 25°C. The secondary used was a Cy3-AffiniPure F(ab')2 fragment goat anti-rabbit monoclonal, used at a 1/200 dilution.