KN-62,CaM kinase II抑制剂. P2X7拮抗剂. (ab120421)
Key features and details
- CaM kinase II inhibitor. P2X7 antagonist.
- CAS Number: 127191-97-3
- Purity: > 97%
- Soluble in DMSO to 100 mM
- Form / State: Solid
- Source: Synthetic
概述
-
产品名称
KN-62,CaM kinase II抑制剂. P2X7拮抗剂. -
描述
CaM kinase II抑制剂. P2X7拮抗剂. -
生物学描述
Cell-permeable, reversible and selective inhibitor of CaM kinase II (IC50 = 500 nM). Interacts with the calmodulin binding site of the enzyme. At higher concentrations reported to inhibit GSK3β, PRAK and MAPKAP-K2. Also potent non-competitive antagonist at the P2X7 receptor (IC50 = 15 nM).
-
纯度
> 97% -
CAS编号
127191-97-3 -
化学结构
性能
-
化学名称
4-[(2S)-2-[(5-Isoquinolinylsulfonyl)methylamino]-3-oxo-3-(4-phenyl-1-piperazinyl)propyl] phenylisoquinolinesulfonic acid ester -
分子量
721.84 -
分子式
C38H35N5O6S2 -
PubChem识别号
5312126 -
存放说明
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解度概述
Soluble in DMSO to 100 mM -
处理
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
-
SMILES
O=C([C@H](Cc3ccc(OS(=O)(=O)c2cccc1cnccc12)cc3)N(C)S(=O)(=O)c5cccc4cnccc45)N6CCN(CC6)c7ccccc7 -
来源
Synthetic
-
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab120421于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Functional Studies |
Use at an assay dependent concentration.
|
说明 |
---|
Functional Studies
Use at an assay dependent concentration. |
图片
-
2D chemical structure image of ab120421, KN-62, CaM kinase II inhibitor. P2X7 antagonist.
-
ab18197 staining PCNA in SK-N-SH cells treated with KN-62 (ab120421), by ICC/IF. Increase in PCNA nuclear expression correlates with increased concentration of KN-62, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120421 (KN-62) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab18197 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
文献 (4)
ab120421 被引用在 4 文献中.
- Wu K et al. Activity- and sleep-dependent regulation of tonic inhibition by Shisa7. Cell Rep 34:108899 (2021). PubMed: 33761345
- Glasgow SD et al. Activity-Dependent Netrin-1 Secretion Drives Synaptic Insertion of GluA1-Containing AMPA Receptors in the Hippocampus. Cell Rep 25:168-182.e6 (2018). PubMed: 30282026
- Jing L et al. Despair-associated memory requires a slow-onset CA1 long-term potentiation with unique underlying mechanisms. Sci Rep 5:15000 (2015). PubMed: 26449319
- Luchkina NV et al. Developmental switch in the kinase dependency of long-term potentiation depends on expression of GluA4 subunit-containing AMPA receptors. Proc Natl Acad Sci U S A 111:4321-6 (2014). PubMed: 24599589