产品名称Anti-KAT13A / SRC1抗体
参阅全部 KAT13A / SRC1 一抗
描述兔多克隆抗体to KAT13A / SRC1
经测试应用适用于: IPmore details
种属反应性与反应: Mouse, Human
预测可用于: Rat, Horse, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Elephant
Recombinant fragment corresponding to Human KAT13A/ SRC1 (C terminal). Immunogen maps to a region between residue 1400 and the C-terminus.
Database link: Q15788
- Nuclear extract from HeLa cells.
存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
存储溶液Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
Concentration information loading...
纯度Immunogen affinity purified
纯化说明Antibodies were affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance of 1.4 at 280 nm equals 1.0 mg of IgG.
- Signal Transduction
- Signaling Pathway
- Nuclear Signaling
- Nuclear Hormone Receptors
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Our Abpromise guarantee covers the use of ab10308 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use a concentration of 1 - 4 µg/ml.|
功能Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Involved in the coactivation of different nuclear receptors, such as for steroids (PGR, GR and ER), retinoids (RXRs), thyroid hormone (TRs) and prostanoids (PPARs). Also involved in coactivation mediated by STAT3, STAT5A, STAT5B and STAT6 transcription factors. Displays histone acetyltransferase activity toward H3 and H4; the relevance of such activity remains however unclear. Plays a central role in creating multisubunit coactivator complexes that act via remodeling of chromatin, and possibly acts by participating in both chromatin remodeling and recruitment of general transcription factors. Required with NCOA2 to control energy balance between white and brown adipose tissues. Required for mediating steroid hormone response. Isoform 2 has a higher thyroid hormone-dependent transactivation activity than isoform 1 and isoform 3.
疾病相关Note=A chromosomal aberration involving NCOA1 is a cause of rhabdomyosarcoma. Translocation t(2;2)(q35;p23) with PAX3 generates the NCOA1-PAX3 oncogene consisting of the N-terminus part of PAX3 and the C-terminus part of NCOA1. The fusion protein acts as a transcriptional activator. Rhabdomyosarcoma is the most common soft tissue carcinoma in childhood, representing 5-8% of all malignancies in children.
序列相似性Belongs to the SRC/p160 nuclear receptor coactivator family.
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAS (PER-ARNT-SIM) domain.
结构域The C-terminal (1107-1441) part mediates the histone acetyltransferase (HAT) activity.
Contains 7 Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. LXXLL motifs 3, 4 and 5 are essential for the association with nuclear receptors. LXXLL motif 7, which is not present in isoform 2, increases the affinity for steroid receptors in vitro.
翻译后修饰Sumoylated; sumoylation increases its interaction with PGR and prolongs its retention in the nucleus. It does not prevent its ubiquitination and does not exert a clear effect on the stability of the protein.
Ubiquitinated; leading to proteasome-mediated degradation. Ubiquitination and sumoylation take place at different sites.
Phosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- bHLHe74 antibody
- Class E basic helix-loop-helix protein 74 antibody
- F SRC 1 antibody
ab10308 Immunoprecipitate KAT13A / SRC1 in human Huh-7 whole cell lysate. 1,000,000 cells were lysed and incubated with primary antibody at 4µg/mg lysate and Protein A matrix for 20 hours at 4°C. For western blotting an undiluted Abcam`s ab2859, Rabbit polyclonal to KAT13A / SRC1 was used.
ab10308 used in Immunoprecipitation.
Total protein input is 1 x 106 cells.Whole cell lysate was prepared from VERO cells expressing recombinant SRC1 protein.
Protein A matrix was used for the Immunoprecipitation step.ab10308 used a 4µg/mg lysate for 16 hours at 4°C.
ab2859 was then used for Western Blot at 1µg/mg lysate.
ab10308 has not yet been referenced specifically in any publications.