Application
Western blot
Sample
Human Cell lysate - whole cell (293T cell)
Loading amount
30 µg
Specification
293T cell
Gel Running Conditions
Reduced Denaturing (4-12% Gradient Gel)
Blocking step
Licor Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: Licor Blocking Buffer
Secondary antibody
Name
Non-Abcam antibody was used: IRDye® 800 anti-mouse antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Dilution
1/5000
Detection
Detection method
Licor
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 100 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Nov 10 2009