重组Anti-JunB抗体[EPR6518] (ab128878)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6518] to JunB
- Suitable for: WB, IP, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-JunB抗体[EPR6518]
参阅全部 JunB 一抗 -
描述
兔单克隆抗体[EPR6518] to JunB -
宿主
Rabbit -
经测试应用
适用于: WB, IP, ICC/IFmore details
不适用于: Flow Cyt or IHC-P -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human JunB (N terminal). The exact sequence is proprietary.
Database link: P17275 -
阳性对照
- WB: MCF7, Human tonsil, Human uterus cancer, mouse uterus, rat lung, rat spleen, HeLa and HACAT lysates ICC/IF: HeLa, and MCF7 cells. IP: HeLa cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
解离常数(KD)
KD = 9.30 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR6518 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab128878于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/200. Predicted molecular weight: 36 kDa.
|
|
IP |
1/10 - 1/100.
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|
ICC/IF |
1/200.
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说明 |
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WB
1/200. Predicted molecular weight: 36 kDa. |
IP
1/10 - 1/100. |
ICC/IF
1/200. |
靶标
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功能
Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[CG]TCA-3'. -
序列相似性
Belongs to the bZIP family. Jun subfamily.
Contains 1 bZIP domain. -
翻译后修饰
Ubiquitinated by ITCH, leading to its degradation. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 3726 Human
- Entrez Gene: 16477 Mouse
- Entrez Gene: 24517 Rat
- Omim: 165161 Human
- SwissProt: P17275 Human
- SwissProt: P09450 Mouse
- SwissProt: P24898 Rat
- Unigene: 25292 Human
see all -
别名
- Activator protein 1 antibody
- AP 1 antibody
- AP1 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling JunB with purified ab128878 at 1:200 dilution (9.1 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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All lanes : Anti-JunB antibody [EPR6518] (ab128878) at 1/200 dilution (Purified)
Lane 1 : Rat lung lysates
Lane 2 : Rat spleen lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 36 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted? -
All lanes : Anti-JunB antibody [EPR6518] (ab128878) at 1/1000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : Mouse uterus lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 36 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted? -
Anti-JunB antibody [EPR6518] (ab128878) at 1/1000 dilution (Purified) + Human uterus lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 36 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted? -
ab128878 (purified) at 1:100 dilution (2 µg) immunoprecipitating JunB in HeLa whole cell lysates.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates 10ug
Lane 2 (+): ab128878 & HeLa whole cell lysates
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab128878 in HeLa whole cell lysateFor western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
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All lanes : Anti-JunB antibody [EPR6518] (ab128878) at 1/1000 dilution (Unpurified)
Lane 1 : MCF-7 lysate
Lane 2 : Human tonsil lysate
Lane 3 : Human uterus cancer lysate
Lane 4 : HeLa lysate
Lane 5 : HACAT lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 36 kDa -
Unpurified ab128878, at a 1/250 dilution, staining JunB in MCF7 (Human breast adenocarcinoma cell line) cells by Immunofluorescence.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab128878 被引用在 9 文献中.
- Zhou C et al. Anxa1 in smooth muscle cells protects against acute aortic dissection. Cardiovasc Res 118:1564-1582 (2022). PubMed: 33757117
- Kuonen F et al. c-FOS drives reversible basal to squamous cell carcinoma transition. Cell Rep 37:109774 (2021). PubMed: 34610301
- Xu M et al. miR-3133 inhibits proliferation and angiogenesis by targeting the JUNB/VEGF pathway in human umbilical vein endothelial cells. Oncol Rep 44:1699-1708 (2020). PubMed: 32945523
- Zhang L et al. Cervical Cancer Cells-Secreted Exosomal microRNA-221-3p Promotes Invasion, Migration and Angiogenesis of Microvascular Endothelial Cells in Cervical Cancer by Down-Regulating MAPK10 Expression. Cancer Manag Res 11:10307-10319 (2019). PubMed: 31849520
- Aichem A et al. The ubiquitin-like modifier FAT10 interferes with SUMO activation. Nat Commun 10:4452 (2019). PubMed: 31575873
- Shirali AS et al. A multi-step transcriptional cascade underlies vascular regeneration in vivo. Sci Rep 8:5430 (2018). PubMed: 29615716
- Yoshitomi Y et al. JunB regulates angiogenesis and neurovascular parallel alignment in mouse embryonic skin. J Cell Sci 130:916-926 (2017). PubMed: 28096474
- Suwei D et al. NLK functions to maintain proliferation and stemness of NSCLC and is a target of metformin. J Hematol Oncol 8:120 (2015). Human . PubMed: 26503334
- Chen H et al. S100A14: novel modulator of terminal differentiation in esophageal cancer. Mol Cancer Res 11:1542-53 (2013). Human . PubMed: 24107296