Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
Sample
Mouse Cell (mouse embryonic stem cells)
Specification
mouse embryonic stem cells
Permeabilization
Yes - 0.1% triton x
Fixative
methanol:acetone 1:1
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking buffer
Dilution
1/100
Additional data
Additional Notes
Blue is DAPI, green is ab124924. PFA fixation did not work with this antibody (results not shown).
Abcam response
Thank you for sharing your data with us. Although ab124924 works well on human samples, we know that this antibody does not detect mouse Integrin alpha 6, which is confirmed here. We hope that this information is useful to our customers.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Jan 02 2014