Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Gel Running Conditions
Reduced Denaturing (10 % gel)
Loading amount
30 µg
Treatment
10 ´M tunicamycin for 30 min, 1h, 2h
Specification
HeLa
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA, TBS, tween 0.1%
Dilution
1/5000
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: Cell Signaling Anti-rabbit IgG, HRP-linked Antibod
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Negative control
Lysate from untreated HeLa cells
Exposure
5 second(s)
Bands
Specific: 110 kDa Non-specific: 70 kDa
Positive control
Lysate from HeLa cells treated with 10 ´M tunicamycin for 30 min, 1h, 2h
Additional data
Additional Notes
Lane 1: ctrl Lane 2: 10 ´M tunicamycin for 30 min Lane 3: 10 ´M tunicamycin for 1h Lane 4: 10 ´M tunicamycin for 2h. Lysis buffer: 1x RIPA (ab156034) supplemented with Protease and Phosphatase Inhibitor Cocktail (ab201119). Discount code: ABTRIAL-EVQCQ
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Tamas Czompoly
Verified customer
提交于 May 19 2017