产品名称Anti-IKB alpha (phospho Y42)抗体[EPR2353]
参阅全部 IKB alpha 一抗
描述兔单克隆抗体[EPR2353] to IKB alpha (phospho Y42)
经测试应用适用于: WB, IPmore details
不适用于: Flow Cyt or IHC-P
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human IKB alpha aa 1-100 (phospho Y42).
Database link: P25963
- Lysate from A431 cells pre-treated with pervanadate.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
纯度Tissue culture supernatant
Our Abpromise guarantee covers the use of ab92685 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 36 kDa.|
功能Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
疾病相关Ectodermal dysplasia, anhidrotic, with T-cell immunodeficiency autosomal dominant
序列相似性Belongs to the NF-kappa-B inhibitor family.
Contains 5 ANK repeats.
翻译后修饰Phosphorylated; disables inhibition of NF-kappa-B DNA-binding activity. Phosphorylation at positions 32 and 36 is prerequisite to recognition by UBE2D3 leading to polyubiquitination and subsequent degradation.
Sumoylated; sumoylation requires the presence of the nuclear import signal. Sumoylation blocks ubiquitination and proteasome-mediated degradation of the protein thereby increasing the protein stability.
Monoubiquitinated at Lys-21 and/or Lys-22 by UBE2D3. Ubiquitin chain elongation is then performed by CDC34 in cooperation with the SCF(FBXW11) E3 ligase complex, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. The resulting polyubiquitination leads to protein degradation. Also ubiquitinated by SCF(BTRC) following stimulus-dependent phosphorylation at Ser-32 and Ser-36.
Deubiquitinated by porcine reproductive and respiratory syndrome virus Nsp2 protein, which thereby interferes with NFKBIA degradation and impairs subsequent NF-kappa-B activation.
细胞定位Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export.
- Information by UniProt
- I kappa B alpha antibody
- I-kappa-B-alpha antibody
- IkappaBalpha antibody
All lanes : Anti-IKB alpha (phospho Y42) antibody [EPR2353] (ab92685) at 1/500 dilution
Lane 1 : lysate from untreated A431 cells
Lane 2 : lysate from A431 cells
pre-treated with pervanadate
Lysates/proteins at 10 µg per lane.
All lanes : HRP conjugated Goat anti-Rabbit IgG at 1/2000 dilution
Predicted band size: 36 kDa
ab92685 has not yet been referenced specifically in any publications.