Application
Western blot
Loading amount
100 µg
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (12%)
Sample
Zebrafish Tissue lysate - whole (muslce (igf1 overexpression in transgenic fish mus)
Specification
muslce (igf1 overexpression in transgenic fish mus
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 28°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5%BSA in TBST
Dilution
1/200
Secondary antibody
Name
Non-Abcam antibody was used: Peroxidase-conjugated Donkey Anti-Goat IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Bands
Specific: about 18kd kDa
Exposure
5 minute(s) and 0 second(s)
Negative control
control fish muscle lysates
Positive control
muscle-overexpression-IGF1 transgenic zebrafish muscle lysates
Additional data
Additional Notes
lane1,2 ,5 control; lane3,4,6 TG(igf1 trangenic fish muscle lysates)
DISCOUNT CODE: [42585]
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Dongliang Li
Verified customer
提交于 Nov 28 2013