Anti-IGF1抗体(ab106836)
Key features and details
- Goat polyclonal to IGF1
- Suitable for: WB, ICC, IHC-P
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-IGF1抗体
参阅全部 IGF1 一抗 -
描述
山羊多克隆抗体to IGF1 -
宿主
Goat -
经测试应用
适用于: WB, ICC, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Dog, Pig -
免疫原
Peptide with sequence
C-RSVRAQRHTD
, from the internal region of human IGF1 sequence according to NP_001104753.1; NP_001104754.1; NP_001104755.1; NP_000609.1. -
阳性对照
- ICC: Human HEK-293 cells; WB: Human uterus tissue lysate; IHC-P: Human spleen tissue.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
-
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab106836于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (2) |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 22 kDa).
1 hour primary incubation is recommended for this product. |
ICC |
Use a concentration of 10 µg/ml.
|
|
IHC-P |
Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
说明 |
---|
WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 22 kDa). 1 hour primary incubation is recommended for this product. |
ICC
Use a concentration of 10 µg/ml. |
IHC-P
Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
-
功能
The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. -
疾病相关
Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation. -
序列相似性
Belongs to the insulin family. -
细胞定位
Secreted. - Information by UniProt
-
数据库链接
- Entrez Gene: 610255 Dog
- Entrez Gene: 3479 Human
- Entrez Gene: 16000 Mouse
- Entrez Gene: 24482 Rat
- Omim: 147440 Human
- SwissProt: P33712 Dog
- SwissProt: P01343 Human
- SwissProt: P05019 Human
see all -
形式
There are 2 isoforms produced by alternative splicing. Isoform 1 also known as: IGF-IB; Isoform 2 also known as: IGF-IA. -
别名
- IBP1 antibody
- IGF I antibody
- IGF IA antibody
see all
图片
-
All lanes : Anti-IGF1 antibody (ab106836) at 0.5 µg/ml
Lane 1 : Human uterus tissue lysate in RIPA buffer
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Predicted band size: 22 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted?Primary incubation for 1 hour at room temperature. Detected by chemiluminescence.
-
Immunocytochemistry analysis of paraformaldehyde fixed HEK-293 (human epithelial cell line from embryonic kidney) cells, permeabilized with 0.15% Triton. Primary incubation for 1 hour at 10 µg/mL followed by Alexa Fluor® 488 secondary antibody at 2 µg/mL. The nuclear stain is DAPI (blue).
Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody (2 µg/mL).
数据表及文件
-
SDS download
-
Datasheet download
文献 (12)
ab106836 被引用在 12 文献中.
- Wu H et al. Engineered adipose-derived stem cells with IGF-1-modified mRNA ameliorates osteoarthritis development. Stem Cell Res Ther 13:19 (2022). PubMed: 35033199
- Choi YJ et al. Ameliorative effect of Abeliophyllum distichum Nakai on benign prostatic hyperplasia in vitro and in vivo. Nutr Res Pract 16:419-434 (2022). PubMed: 35919286
- Zeng N et al. Sexual dimorphic effects of igf1 deficiency on metabolism in zebrafish. Front Endocrinol (Lausanne) 13:879962 (2022). PubMed: 35966057
- Xia H et al. Yi-Qi-Jian-Pi-Xiao-Yu-Xie-Zhuo Formula Improves Muscle Atrophy via Modulating the IGF-1/PI3K/Akt Signaling Pathway in 5/6 Nephrectomized Rats. Front Pharmacol 12:624303 (2021). PubMed: 33986663
- Ai D & Yu F LncRNA DNM3OS promotes proliferation and inhibits apoptosis through modulating IGF1 expression by sponging MiR-126 in CHON-001 cells. Diagn Pathol 14:106 (2019). PubMed: 31526393