Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Human embryonic lung - 7 weeks post conception)
Permeabilization
Yes - 10 min 0.3% triton post-sectioning
Specification
Human embryonic lung - 7 weeks post conception
Blocking step
1% BSA, 5% normal donkey serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative
Paraformaldehyde
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA, 5% NDS, 0.1% Triton X-100
Dilution
1/200
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: anti-rabbit 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
Additional data
Additional Notes
Worked well for frozen sections. ID2 (green) is specific for tip epithelium.
There is also some mesenchymal staining which we suspect is real.
Image is co-stained with E-cadherin (white) to show epithelium.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MS. Emma Rawlins
Verified customer
提交于 May 24 2017