Application
Western blot
Sample
Human Cell lysate - whole cell (Cancer cell lines total lysate)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
20 µg
Specification
Cancer cell lines total lysate
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST + 1%BSA
Dilution
1/500
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Goat anti-mouse IgG-HRP sc-2031
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ecl
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: ~95 kDa Non-specific: ~60 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Dec 07 2015