Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Mesenchymal stem cell)
Specification
Mesenchymal stem cell
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
Other product details
Dilution
1/100
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: 10% horse serum
Secondary antibody
Name
Non-Abcam antibody was used: Southern Biotech IgG2a
Host species: Mouse
Clonality: Monoclonal
Conjugation: TRITC
Host species: Mouse
Clonality: Monoclonal
Conjugation: TRITC
Dilution
1/100
Additional data
Additional Notes
Very good staining was obtained using TRITC conjugated secondary. Antibody was used as a marker for mesenchymal stem cell classification of both bone marrow and lamina propria derived mesenchymal stem cells. The image show ICAM1 in red co-stained alongside CD90 in green. Staining was vivid and reproducable accross multiple replicates of both cell types
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Steven Johnstone
Verified customer
提交于 Sep 05 2012