We tested this product on pig brain tissue (thawed from snapfrozen in liquid nitrogen), since we were informed that there is a 95% homology between human and pig SHH protein sequences. Since no information was available for which diluent to use, we found diluent B most appropiate and used this. We followed the protocol step by step.
We had no problem getting acceptable results from the ELISA and we will now proceed to order yet another kit for the remaining brain samples to be analyzed.
Although the kit worked fine, the manual is somewhat messy at places (e.g. the peculiar dilutions used in the standard curve could use some explanation), but nevertheless, the kit seems to work fine in pig brain tissue.
We had no problem getting acceptable results from the ELISA and we will now proceed to order yet another kit for the remaining brain samples to be analyzed.
Although the kit worked fine, the manual is somewhat messy at places (e.g. the peculiar dilutions used in the standard curve could use some explanation), but nevertheless, the kit seems to work fine in pig brain tissue.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Nov 21 2013