人Perforin ELISA试剂盒(PRF1) (ab46068)
Key features and details
- Sensitivity: 40 pg/ml
- Range: 62.5 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人Perforin ELISA试剂盒(PRF1) -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% A 8 416pg/ml 20 5% B 8 674pg/ml 25 4% C 8 984pg/ml 24 3% 批次间 样品 n Mean SD CV% A 9 580pg/ml 15 3% B 9 665pg/ml 31 5% C 9 1004pg/ml 45 5% -
样品类型
Cell culture supernatant -
检测类型
Sandwich (quantitative) -
灵敏度
< 40 pg/ml -
范围
62.5 pg/ml - 2000 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 71 % - % -
检测时间
2h 45m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s Perforin (PRF1) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Perforin in supernatant and buffered solutions.
A monoclonal antibody specific for Perforin has been coated onto the wells of the microtiter strips provided. Samples, including standards of known Perforin concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for Perforin are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of Perforin present in the samples.
This kit will recognize both endogenous and recombinant Human Perforin.
Please note this kit is not suitable for use with serum and plasma. -
平台
Microplate
性能
-
存放说明
Store at +4°C. Please refer to protocols. -
组件 标识符 2 x 96 tests 1 x 96 tests 1 x 96 tests 2 x 96 tests 1X Standard Diluent Buffer 2 x 25ml 1 x 25ml 1 x 25ml 2 x 25ml 200X Wash Buffer White 2 x 10ml 1 x 10ml 1 x 10ml 2 x 10ml Biotinylated anti PRF1 Red 2 x 0.4ml 1 x 0.4ml 1 x 0.4ml 2 x 0.4ml Biotinylated Antibody Diluent Red 1 x 13ml 1 x 7.5ml 1 x 7.5ml 1 x 13ml Chromogen TMB Substrate Solution 1 x 24ml 1 x 11ml 1 x 11ml 1 x 24ml HRP Diluent Red 1 x 23ml 1 x 12ml 1 x 12ml 1 x 23ml PRF1 Microplate (12 x 8 well strips) 2 units 1 unit 1 unit 2 units PRF1 Standard (Lyophilized) Yellow 4 vials 2 vials 2 vials 4 vials Stop Reagent Black 2 x 11ml 1 x 11ml 1 x 11ml 2 x 11ml Streptavidin-HRP 4 x 5µl 2 x 5µl 2 x 5µl 4 x 5µl -
研究领域
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功能
Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes. -
疾病相关
Defects in PRF1 are the cause of hemophagocytic lymphohistiocytosis familial type 2 (FHL2) [MIM:603553]; also known as HPLH2. Familial hemophagocytic lymphohistiocytosis (FHL) is a genetically heterogeneous, rare autosomal recessive disorder. It is characterized by immune dysregulation with hypercytokinemia and defective natural killer cell function. The clinical features of the disease include fever, hepatosplenomegaly, cytopenia, hypertriglyceridemia, hypofibrinogenemia, and neurological abnormalities ranging from irritability and hypotonia to seizures, cranial nerve deficits, and ataxia. Hemophagocytosis is a prominent feature of the disease, and a non-malignant infiltration of macrophages and activated T lymphocytes in lymph nodes, spleen, and other organs is also found. -
序列相似性
Belongs to the complement C6/C7/C8/C9 family.
Contains 1 C2 domain.
Contains 1 EGF-like domain.
Contains 1 MACPF domain. -
结构域
The C2 domain mediates calcium-dependent binding to lipid membranes. A subsequent conformation change leads to membrane insertion of beta-hairpin structures and pore formation. The pore is formed by transmembrane beta-strands. -
翻译后修饰
N-glycosylated. -
细胞定位
Cytoplasmic granule lumen. Secreted. Cell membrane. Endosome lumen. Stored in cytoplasmic granules of cytolytic T-lymphocytes and secreted into the cleft between T-lymphocyte and target cell. Inserts into the cell membrane of target cells and forms pores. Membrane insertion and pore formation requires a major conformation change. May be taken up via endocytosis involving clathrin-coated vesicles and accumulate in a first time in large early endosomes. - Information by UniProt
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别名
- Cytolysin
- FLH2
- HPLH2
see all -
数据库链接
- Entrez Gene: 5551 Human
- Omim: 170280 Human
- SwissProt: P14222 Human
- Unigene: 2200 Human
图片
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab46068 被引用在 7 文献中.
- Chu Y et al. Combinatorial immunotherapy of N-803 (IL-15 superagonist) and dinutuximab with ex vivo expanded natural killer cells significantly enhances in vitro cytotoxicity against GD2+ pediatric solid tumors and in vivo survival of xenografted immunodeficient NSG mice. J Immunother Cancer 9:N/A (2021). PubMed: 34244307
- Sasawatari S et al. Blockade of N-Glycosylation Promotes Antitumor Immune Response of T Cells. J Immunol 204:1373-1385 (2020). PubMed: 31969386
- Hu G et al. An IL6-Adenosine Positive Feedback Loop between CD73+ γδTregs and CAFs Promotes Tumor Progression in Human Breast Cancer. Cancer Immunol Res 8:1273-1286 (2020). PubMed: 32847938
- Sevim H et al. Bone marrow derived mesenchymal stem cells ameliorate inflammatory response in an in vitro model of familial hemophagocytic lymphohistiocytosis 2. Stem Cell Res Ther 9:198 (2018). PubMed: 30021624
- Wang Y et al. NKG2D modulates aggravation of liver inflammation by activating NK cells in HBV infection. Sci Rep 7:88 (2017). PubMed: 28273905
- Sarkar S et al. Dynamic Analysis of Human Natural Killer Cell Response at Single-Cell Resolution in B-Cell Non-Hodgkin Lymphoma. Front Immunol 8:1736 (2017). ELISA . PubMed: 29312292
- Freeman LM et al. Myeloma-induced alloreactive T cells arising in myeloma-infiltrated bones include double-positive CD8+CD4+ T cells: evidence from myeloma-bearing mouse model. J Immunol 187:3987-96 (2011). PubMed: 21908738