The human insulin ELISA kit (ab200011) is easy to work with and reproducible. We used the kit with insulin-secreting human cell lines following the manufacturer's protocol. Cells were treated with glucose to detect insulin secretion and cells were lysed to detect the insulin content. The kit worked perfectly when detecting high content of insulin. However, when the insulin content is low in the samples, more repeats may be needed as the standard curve at low concentration was slightly non-liner and variable in the three repeats we conducted. Overall, the kit works well in detecting insulin and we are pleased with the results.