人C Reactive蛋白ELISA试剂盒(CRP) (ab99995)
Key features and details
- Sensitivity: 2 pg/ml
- Range: 34.29 pg/ml - 25000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人C Reactive蛋白ELISA试剂盒(CRP)
参阅全部 C Reactive Protein 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 2 pg/ml -
范围
34.29 pg/ml - 25000 pg/ml -
回收率
> 100 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 106.2 95% - 116% Serum 116.7 106% - 127% Plasma 102.2 93% - 112% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s C Reactive Protein (CRP) Human ELISA (Enzyme- Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CRP in serum, plasma, cell culture supernatants.
This assay employs an antibody specific for Human CRP coated on a 96-well plate. Standards and samples are pipetted into the wells and CRP present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CRP antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CRP bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get results in 90 minutes with Human C Reactive Protein ELISA Kit (ab181416) from our SimpleStep ELISA® range.
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说明
Optimization may be required with urine samples.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 2 x 15ml CRP Microplate (12 x 8 well strips) 1 unit Biotinylated anti-Human CRP 2 vials Recombinant Human CRP Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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功能
Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. -
组织特异性
Found in plasma. -
序列相似性
Belongs to the pentaxin family.
Contains 1 pentaxin domain. -
细胞定位
Secreted. - Information by UniProt
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别名
- Pentraxin 1, short
- C reactive protein
- C reactive protein pentraxin related
see all -
数据库链接
- Entrez Gene: 1401 Human
- Omim: 123260 Human
- SwissProt: P02741 Human
- Unigene: 709456 Human
- Unigene: 76452 Human
图片
数据表及文件
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SDS download
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Datasheet download
文献 (33)
ab99995 被引用在 33 文献中.
- Minas TZ et al. Serum proteomics links suppression of tumor immunity to ancestry and lethal prostate cancer. Nat Commun 13:1759 (2022). PubMed: 35365620
- Barcikowska Z et al. Effect of Manual Therapy Compared to Ibuprofen on Primary Dysmenorrhea in Young Women-Concentration Assessment of C-Reactive Protein, Vascular Endothelial Growth Factor, Prostaglandins and Sex Hormones. J Clin Med 11:N/A (2022). PubMed: 35628817
- Kared H et al. Immune responses in Omicron SARS-CoV-2 breakthrough infection in vaccinated adults. Nat Commun 13:4165 (2022). PubMed: 35851055
- Han D et al. Upregulated lncRNA-NEF predicts recurrence and poor treatment outcomes of ankylosing spondylitis. Immun Inflamm Dis 10:e627 (2022). PubMed: 35894706
- Ferguson DP et al. C-reactive protein and telomerase reverse transcriptase (TERT) associate with chronic disease markers in a sample from low-income neighborhoods in Detroit, Michigan. Sports Med Health Sci 4:275-279 (2022). PubMed: 36600969