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Although this kit has not been tested in cell or tissue lysates I want to test it with cells removed from the matrix. Are there any lysis buffers that I should avoid that may interfere with the kit?
Asked on Sep 25 2013
While this kit as not been tested with tissue or cell lysates, in general, a lysis buffer containg a low concentration of triton-X-100 or SP-40 (less than 1 %) will not affect the assay.
Abcam Scientific Support
回复于 Sep 25 2013