人Albumin ELISA试剂盒(ab108787)
Key features and details
- Sensitivity: 0.28 µg/ml
- Range: 1.5 µg/ml - 25 µg/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
概述
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产品名称
人Albumin ELISA试剂盒
参阅全部 Albumin 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 3.3% 批次间 样品 n Mean SD CV% Overall 10.6% -
样品类型
Serum, Plasma -
检测类型
Competitive -
灵敏度
= 0.28 µg/ml -
范围
1.5 µg/ml - 25 µg/ml -
回收率
98 %
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检测时间
2h 00m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s Albumin Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the measurement of albumin in Human plasma and serum.
An albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated albumin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of albumin captured in plate.
Get results in 90 minutes with Human Serum Albumin ELISA Kit (ab179887) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 1 x 30ml 3X Biotinylated Human Albumin (Lyophilized) 1 vial Albumin Microplate (12 x 8 well strips) 1 unit Albumin Standard 1 vial Chromogen Substrate 1 x 7ml Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究领域
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功能
Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. -
组织特异性
Plasma. -
疾病相关
Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population. -
序列相似性
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
翻译后修饰
Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
Glycated in diabetic patients.
Phosphorylation sites are present in the extracelllular medium.
Acetylated on Lys-223 by acetylsalicylic acid. -
细胞定位
Secreted. - Information by UniProt
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别名
- alb
- ALBU_HUMAN
- Albumin (32 AA)
see all -
数据库链接
- Entrez Gene: 213 Human
- Omim: 103600 Human
- SwissProt: P02768 Human
- Unigene: 418167 Human
- Unigene: 592379 Human
相关产品
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SimpleStep ELISA kits
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab108787 被引用在 3 文献中.
- Farooqi HMU et al. Real-time monitoring of liver fibrosis through embedded sensors in a microphysiological system. Nano Converg 8:3 (2021). PubMed: 33528697
- Kim YS et al. Gravity-Based Flow Efficient Perfusion Culture System for Spheroids Mimicking Liver Inflammation. Biomedicines 9:N/A (2021). PubMed: 34680487
- Lee MJ et al. Free 25-Hydroxyvitamin D Concentrations in Cystic Fibrosis. Am J Med Sci 350:374-9 (2015). PubMed: 26512456