Application
ChIP-sequencing
Sample
Mouse Tissue lysate - whole (Brain)
Specification
Brain
Other product details
Incubation time
8 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/300
Secondary antibody
Secondary antibody
None used
Additional data
Additional Notes
ChIP-seq reads were aligned against the mm10 mouse genome, and wig files were generated and uploaded to the UCSC genome browser.
Image shows the total level of signal (coverage of reads after alignement) in three different regions, a highly repressed one (Olfr cluster) and two transcriptionally active regions (Tuba1a and Gapdh).
For primary antibody incubation the dilution buffer composition was: 50 mM Hepes pH 8, 140 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0,1% NaDOC, 0,1% SDS 1x Protease inhibitors cocktail
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jul 05 2018