Application
Western blot
Sample
Saccharomyces cerevisiae Cell lysate - whole cell (no case)
Loading amount
2 µg
Specification
no case
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Dilution
1/500
Incubation time
16 hour(s) and 0 minute(s) · Diluent: TBS+5% Tween+5%BSA
Secondary antibody
Name
Non-Abcam antibody was used: goat antirabbit _HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/30000
Detection
Detection method
ECL
Exposure
20 second(s)
Bands
Specific: none kDa Non-specific: 18, 21, 35, 50, 60, 85 kDa
Positive control
H3 western blot with ab 1791 for loading of H3 (left blot) and ab9048 K36me1 Abcam for
specificity of phenotypes (not shown) on WT and set2 KO strains.
specificity of phenotypes (not shown) on WT and set2 KO strains.
Negative control
Set2 KO strain.
Additional data
Additional Notes
lines loading from right to left:
1) ume6 KO
2) cti6 KO
3) EAF3 KO
4) set2 KO
5) Wild type
Left gel: ab1791
Right gel: ab9048
1) ume6 KO
2) cti6 KO
3) EAF3 KO
4) set2 KO
5) Wild type
Left gel: ab1791
Right gel: ab9048
Abcam response
This negative Abreview is currently being investigated by Abcam Technical Support. Please check here for an update response from Abcam.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Oct 23 2007