Application
ChIP
Sample
Mouse Tissue lysate - nuclear (Hippocampus)
Negative control
Unspecific IgG
Specification
Hippocampus
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde in PBS
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde in PBS
Positive control
2 concentration of the antibody: 1/500 and 1/250
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Dilution Buffer (Additional notes)
Dilution
1/500
Additional data
Additional Notes
Dilution Buffer:
0,01% SDS, 1,1% Triton X-100, 1,2 mM EDTA pH8, 16,7 mM Tris pH 8, 167 mM NaCl
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提交于 Aug 31 2018