Application
Western blot
Sample
Schmidtea mediterranea Tissue lysate - whole (Whole worm lysate)
Gel Running Conditions
Reduced Denaturing (4-12%BT-gel)
Loading amount
10 µg
Specification
Whole worm lysate
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/10000
Detection
Detection method
ECL
Exposure
15 minute(s) and 0 second(s)
Bands
Specific: 15KDA kDa
Positive control
HELA NUCLEAR EXTRACT
Additional data
Additional Notes
First Lane HELA -
Second Lane S. Mediterranean whole worm lysate
Bands appear to be around 14-16KDA. Non-specific bands are observed in both.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Anish Dattani
Verified customer
提交于 Feb 22 2018