Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton X-100
Specification
HeLa
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS
Dilution
1/200
Secondary antibody
Dilution
1/200
Name
Abcam antibody:Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed
Additional data
Additional Notes
Asynchronous cells were paraformaldehyde-fixed, permeablized, immunofluorescently labelled and counterstained with DAPI. Under these conditions, cells exhibited a nuclear-enriched, focal staining pattern. MeOH fixed samples were also evaluated, but did not produce the expected staining pattern.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Kirk Mcmanus
Verified customer
提交于 Jul 26 2017