Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (15%)
Sample
Rat Cell lysate - nuclear (vascular smooth muscle cells)
Specification
vascular smooth muscle cells
Treatment
80 uM tert-butyl BHP
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: anti rabbit
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL
Bands
Specific: 15 kDa Non-specific: 25 kDa
Exposure
1 minute(s) and 0 second(s)
Additional data
Additional Notes
VERY GOOD ANTIBODY FOR DETECTION OF TOTAL PROTEIN
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Jun 24 2013