Anti-Hes1抗体[OTI2D2] (ab119776)
Key features and details
- Mouse monoclonal [OTI2D2] to Hes1
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Hes1抗体[OTI2D2]
参阅全部 Hes1 一抗 -
描述
小鼠单克隆抗体[OTI2D2] to Hes1 -
宿主
Mouse -
经测试应用
适用于: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human, Recombinant fragment -
免疫原
Recombinant full length Human Hes1 produced in HEK293T cells (NP_005515).
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阳性对照
- WB: HEK293T cell lysate transfected with pCMV6-ENTRY Hes1 cDNA. ICC/IF: MCF7 cell lysates; COS7 cells transiently transfected by pCMV6-ENTRY Hes1 cDNA. Flow Cyt (Intra): Jurkat cells. IHC-P: Human thyroid, thyroid carcinoma and bladder carcinoma tissues.
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常规说明
Dilute in PBS (pH7.3) before use.
This product was changed from ascites to tissue culture supernatant on the 4th of June 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading...
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纯度
Tissue culture supernatant -
纯化说明
Purified from TCS. -
克隆
单克隆 -
克隆编号
OTI2D2 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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KO cell lines
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab119776于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
1/100.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 30 kDa.
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IHC-P |
Use at an assay dependent concentration.
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ICC/IF | (2) |
Use at an assay dependent concentration.
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说明 |
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Flow Cyt (Intra)
1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 30 kDa. |
IHC-P
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
靶标
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功能
Transcriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity. -
序列相似性
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 Orange domain. -
结构域
Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).
The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.
The bHLH, as well as cooperation between the central Orange domain and the C-terminal WRPW motif, is required for transcriptional repressor activity. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 3280 Human
- Omim: 139605 Human
- SwissProt: Q14469 Human
- Unigene: 250666 Human
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别名
- bHLHb39 antibody
- C-HAIRY1 antibody
- c-hairy1A antibody
see all
图片
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All lanes : Anti-Hes1 antibody [OTI2D2] (ab119776) at 1/2000 dilution
Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY Hes1 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 30 kDaHEK293T cell lysates were generated from transient transfection of the cDNA clone (RC211709)
This image was generated using the ascites version of the product.
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ab119776, at 1/150 dilution, staining Hes1 in paraffin-embedded Human bladder carcinoma tissue by Immunohistochemistry.
This image was generated using the ascites version of the product.
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ab119776, at 1/150 dilution, staining Hes1 in paraffin-embedded Human thyroid carcinoma tissue by Immunohistochemistry.
This image was generated using the ascites version of the product.
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ab119776, at 1/150 dilution, staining Hes1 in paraffin-embedded Human thyroid tissue by Immunohistochemistry.
This image was generated using the ascites version of the product.
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ab119776, at 1/100 dilution, staining Hes1 in COS7 cells transiently transfected with pCMV6-ENTRY Hes1 cDNA by Immunofluorescence.
This image was generated using the ascites version of the product.
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ab119776, at 1/100 dilution, staining Hes1 (red) in Jurkat cells, compared to a nonspecific negative control antibody,(blue) by Flow cytometry (Intracellular).
This image was generated using the ascites version of the product.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (13)
ab119776 被引用在 13 文献中.
- Chesnais F et al. High-content image analysis to study phenotypic heterogeneity in endothelial cell monolayers. J Cell Sci 135:N/A (2022). PubMed: 34982151
- Zheng Y et al. Nogo-B promotes angiogenesis and improves cardiac repair after myocardial infarction via activating Notch1 signaling. Cell Death Dis 13:306 (2022). PubMed: 35383153
- Liu CF et al. Whole-Transcriptome Profiling of Human Heart Tissues Reveals the Potential Novel Players and Regulatory Networks in Different Cardiomyopathy Subtypes of Heart Failure. Circ Genom Precis Med 14:e003142 (2021). PubMed: 33517678
- Tao ZS et al. Hydrogel contained valproic acid accelerates bone-defect repair via activating Notch signaling pathway in ovariectomized rats. J Mater Sci Mater Med 33:4 (2021). PubMed: 34940936
- Fan J et al. Targeting the Notch and TGF-ß signaling pathways to prevent retinal fibrosis in vitro and in vivo. Theranostics 10:7956-7973 (2020). PubMed: 32724452