抗乙型肝炎病毒Core Antigen抗体[10E11] (ab8639)
Key features and details
- Mouse monoclonal [10E11] to Hepatitis B Virus Core Antigen
- Suitable for: ICC/IF, WB, IP, ELISA, IHC-P, IHC-Fr
- Reacts with: Hepatitis B virus
- Isotype: IgG1
概述
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产品名称
抗乙型肝炎病毒Core Antigen抗体[10E11]
参阅全部 Hepatitis B Virus Core Antigen 一抗 -
描述
小鼠单克隆抗体[10E11] to乙型肝炎病毒Core Antigen -
宿主
Mouse -
特异性
This antibody reacts with HBV Core Antigen (amino acid residues 1-10). Ab8639 should recognize both the precoreprotein and core protein.
Ab8639 was raised against serotype ayw but will work with all other genotypes.
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经测试应用
适用于: ICC/IF, WB, IP, ELISA, IHC-P, IHC-Frmore details -
种属反应性
与反应: Hepatitis B virus -
免疫原
Tissue, cells or virus corresponding to Hepatitis B virus Hepatitis B Virus Core Antigen. Purified Denatured Hepatitis B Core Antigen
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表位
aa positions 1-10 -
常规说明
This product is raised in the same, but denatured, HBcAg protein sequence as ab8637, hence have more robust activity in denaturing western blots/IF etc.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading...
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纯度
Tissue culture supernatant -
纯化说明
The bioreactor harvest was dialyzed against PBS, pH 7.4, BSA was added, and the prep was filter-sterilized -
克隆
单克隆 -
克隆编号
10E11 -
骨髓瘤
Sp2/0 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
应用 | Ab评论 | 说明 |
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ICC/IF |
1/100.
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WB |
1/1000.
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|
IP |
Use at an assay dependent concentration.
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|
ELISA |
Use at an assay dependent concentration.
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IHC-P |
1/100.
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IHC-Fr |
1/100.
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说明 |
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ICC/IF
1/100. |
WB
1/1000. |
IP
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
IHC-P
1/100. |
IHC-Fr
1/100. |
靶标
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相关性
Hepatitis B Virus Core Antigen (HBcAg) is part of the infectious virion containing an inner "core particle" enclosing the viral genome. The icosahedral core particle contains 180 or 240 copies of the core protein. HBcAg is one of the three major clinical antigens of hepatitis B virus but disappears early in the course of infection. The hepatitis B virus core antigen (HBcAg) is a highly immunogenic subviral particle and functions as both a T-cell-dependent and a T-cell-independent antigen. Therefore, HBcAg may be a promising candidate target for therapeutic vaccine control of chronic HBV infection. -
细胞定位
Capsid protein: Virion. Host cytoplasm, hepatocyte nucleus. -
数据库链接
- Entrez Gene: 2828293 Hepatitis B virus
- Entrez Gene: 944568 Hepatitis B virus
- SwissProt: P03146 Hepatitis B virus
- SwissProt: P03147 Hepatitis B virus
- SwissProt: P03148 Hepatitis B virus
- SwissProt: P0C573 Hepatitis B virus
- SwissProt: P69706 Hepatitis B virus
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别名
- C antibody
- Capsid protein antibody
- Core and e antigen antibody
see all
实验方案
数据表及文件
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Datasheet download
文献 (16)
ab8639 被引用在 16 文献中.
- Lin J et al. Bay41-4109-induced aberrant polymers of hepatitis b capsid proteins are removed via STUB1-promoted p62-mediated macroautophagy. PLoS Pathog 18:e1010204 (2022). PubMed: 35030230
- Colant N et al. Escherichia Coli-Based Cell-Free Protein Synthesis for Iterative Design of Tandem-Core Virus-Like Particles. Vaccines (Basel) 9:N/A (2021). PubMed: 33669126
- Jiao Q et al. NLRX1 can counteract innate immune response induced by an external stimulus favoring HBV infection by competitive inhibition of MAVS-RLRs signaling in HepG2-NTCP cells. Sci Prog 104:368504211058036 (2021). PubMed: 34825857
- Shu W et al. Regulation of Molecular Chaperone GRP78 by Hepatitis B Virus: Control of Viral Replication and Cell Survival. Mol Cell Biol 40:N/A (2020). PubMed: 31712392
- Peyret H et al. Covalent protein display on Hepatitis B core-like particles in plants through the in vivo use of the SpyTag/SpyCatcher system. Sci Rep 10:17095 (2020). PubMed: 33051543