Anti-HUS1抗体(ab96297)
Key features and details
- Rabbit polyclonal to HUS1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-HUS1抗体
参阅全部 HUS1 一抗 -
描述
兔多克隆抗体to HUS1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Rat, Cow, Pig -
免疫原
Recombinant protein fragment containing a sequence corresponding to a region within amino acids 1 - 243 of Human HUS1 (AAH07013).
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阳性对照
- 293T cell lysate, CA922 xenograft
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab96297于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 32 kDa.
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IHC-P |
1/100 - 1/500.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 32 kDa. |
IHC-P
1/100 - 1/500. |
靶标
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功能
Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. -
组织特异性
Ubiquitous. -
序列相似性
Belongs to the HUS1 family. -
细胞定位
Nucleus. Cytoplasm. In discrete nuclear foci upon DNA damage. According to PubMed:14500360, localized also in the cytoplasm. DNA damage induces its nuclear translocation. Shuttles between the nucleus and the cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 514142 Cow
- Entrez Gene: 3364 Human
- Entrez Gene: 100192318 Pig
- Entrez Gene: 498411 Rat
- Omim: 603760 Human
- SwissProt: O60921 Human
- Unigene: 152983 Human
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别名
- Checkpoint protein HUS1 antibody
- hHUS1 antibody
- HUS1 (S. pombe) checkpoint homolog antibody
see all
图片
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab96297 被引用在 4 文献中.
- Lindner AK et al. HUS1 as a Potential Therapeutic Target in Urothelial Cancer. J Clin Med 11:N/A (2022). PubMed: 35456300
- Pillay N et al. DNA Replication Vulnerabilities Render Ovarian Cancer Cells Sensitive to Poly(ADP-Ribose) Glycohydrolase Inhibitors. Cancer Cell 35:519-533.e8 (2019). PubMed: 30889383
- Osorio-Zambrano WF & Davey S Chk1 Activation Protects Rad9A from Degradation as Part of a Positive Feedback Loop during Checkpoint Signalling. PLoS One 10:e0144434 (2015). PubMed: 26658951
- Ding G et al. HIF1-regulated ATRIP expression is required for hypoxia induced ATR activation. FEBS Lett 587:930-5 (2013). PubMed: 23454212