存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
存储溶液Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration information loading...
纯度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab109706 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 12 kDa.|
|IHC-P||Use a concentration of 3.75 µg/ml.|
功能HMG-I/Y bind preferentially to the minor groove of A+T rich regions in double stranded DNA. It is suggested that these proteins could function in nucleosome phasing and in the 3'-end processing of mRNA transcripts. They are also involved in the transcription regulation of genes containing, or in close proximity to A+T-rich regions.
疾病相关Note=A chromosomal aberration involving HMGA1 is found in pulmonary chondroid hamartoma. Translocation t(6;14)(p21;q23-24) with RAD51L1.
序列相似性Belongs to the HMGA family.
Contains 3 A.T hook DNA-binding domains.
翻译后修饰Constitutively phosphorylated on two or three sites. Phosphorylated upon DNA damage, probably by ATM or ATR. Hyperphosphorylated at early stages of apoptosis, followed by dephosphorylation and methylation, which coincides with chromatin condensation. Isoform HMG-Y can be phosphorylated by HIPK2.
HMG-Y is not methylated.
Methylation at Arg-58 is mutually exclusive with methylation at Arg-60.
- Information by UniProt
- High mobility group AT hook 1 antibody
- High mobility group AT-hook protein 1 antibody
- High mobility group protein A1 antibody
Anti-HMGA1a / HMGA1b antibody (ab109706) at 0.5 µg/ml (incubation time: 1 hour) + Human pancreas lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 12 kDa
ab109706 at 3.75 µg/ml staining formalin-fixed, paraffin-embedded Human placenta tissue.
ab109706 at 3.75 µg/ml staining formalin-fixed, paraffin-embedded Human skin tissue.
ab109706 has not yet been referenced specifically in any publications.