Application
Western blot
Sample
Human Cell lysate - nuclear (MCF7)
Loading amount
25 µg
Specification
MCF7
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Milk 1% TBS T
Secondary antibody
Name
Non-Abcam antibody was used: DAKO anti Mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Detection
Detection method
ECL
Exposure
20 minute(s) and 0 second(s)
Bands
Specific: 60 kDa
Positive control
Cells under hipoxia conditions that promotes HIF 1a expression.
Additional data
PubMed ID
19955572: View
Additional Notes
MCF-7 cells were cultured under normoxic (N) or hypoxic(H) conditions for72h or in normoxic condition withTGF-b1(5 ng/ml) for the same length of time.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jan 21 2013