Application
ChIP
Sample
Mouse Cell lysate - whole cell (Liver, Hepatoma)
Negative control
Normal mouse IgG
Specification
Liver, Hepatoma
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 15 minute(s) and 0 second(s)
Specification of the cross-linking agent: formaldehyde
Duration of cross-linking step: 15 minute(s) and 0 second(s)
Specification of the cross-linking agent: formaldehyde
Positive control
Anti RNA polymerase II
Other product details
Incubation time
1 hour(s) and 30 minute(s) · Temperature: 25°C · Diluent: Antibody buffer / Sigma Imprint ChIP Kit
Dilution
1/100
Additional data
Additional Notes
Sigma Imprint ChIP Kit is used. Everything is within the range suggested by its protocol. The data are normalized to 10% Input. The difference of HDAC2 binding between two loci of one gene is compared.
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提交于 Sep 18 2008