Application
Western blot
Excellent specificity.
Excellent sensitivity.
WB conditions:
- Cell lisates: 20 μg protein
- non-reducing
- Primary antibody: Rat anti-Lamp1, clon 1D4B. Hybridoma Bank. Dilution 1:1000
- Secondary antibody: 1:6000
- Developer: ECL Prime, Amersham, GE Helathcare Life Sciences, Product code RPN2232
- Exposure time: 1 second, 3th film out 4
Excellent sensitivity.
WB conditions:
- Cell lisates: 20 μg protein
- non-reducing
- Primary antibody: Rat anti-Lamp1, clon 1D4B. Hybridoma Bank. Dilution 1:1000
- Secondary antibody: 1:6000
- Developer: ECL Prime, Amersham, GE Helathcare Life Sciences, Product code RPN2232
- Exposure time: 1 second, 3th film out 4
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Feb 01 2016