为了使您在Abcam官网的浏览体验更顺畅，请使用最新版本的浏览器比如 Google Chrome
Full length protein corresponding to Mouse IgG.
Our Abpromise guarantee covers the use of ab216776 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|In-Cell ELISA||1/200 - 1/800.|
|WB||1/5000 - 1/25000.|
This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with ab128874 overnight at 4°C. Antibody binding was detected using the Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773. Loading control to GAPDH ab8245 antibody binding was detected using the Goat anti-Mouse IgG H&L (IRDye® 680RD) (ab216776) at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
Merged signal (red and green). Green Anti-Brd4 antibody [EPR5150(2)] ab128874 observed at 150 kDa using Goat anti-Rabbit IgG H&L (IRDye® 800CW)-ab216773 as secondary antibody. Red - Anti-GAPDH antibody loading control, ab8245, observed at 37 kDa,
Excitation and emission spectra of IRDye® 680RD Ex: 680, Em:694.
ab216776 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"