Application
Flow Cytometry
Sample
Human Cell (ovarian cancer (OVCAr-3))
Permeabilization
Yes - Ethanol 70%, 30 min at -20
Specification
ovarian cancer (OVCAr-3)
Other product details
Incubation time
30 minute(s)
Dilution
1/100
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Goat anti Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Fluorescein
Host species: Goat
Clonality: Polyclonal
Conjugation: Fluorescein
Additional data
Additional Notes
DISCOUNT CODE: 95695
Gpx was highly positive with this Ab by western but no results for flow-cytoemtry (attched figure). Black=Isotype control, Green=tested antibody
Abcam response
Thank you for your valuable feedback and for participating in our Abtrial testing program. We are sorry to hear that this antibody has not given you good results in Flow cytometry. This antibody has not been tested with this application before, and it is possible that it may not be suitable for use.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Nov 25 2016