Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (cerebellum)
Specification
cerebellum
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Other product details
Dilution
1/200
Incubation time
2 hour(s) and 0 minute(s) · Diluent: TBS/BSA/azide
Secondary antibody
Name
Non-Abcam antibody was used: anti rabbit IgGs
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200
Additional data
Additional Notes
Submitted composite image shows localisation of this protein apparently exclusive to the molecular layer of the cerebellum upper image (low power).
In the lower image (higher power), the molecular layer positivity appears to be compartmentalised/restricted....
I am not very familar with AMPA GluR1 distribution in the cerebellum nor the relationships with component cells/fibres, so I ask if the positivity is not only localised to Climbing fibres but, also to Bergmann glia?
Also, there is a concentration of positivity closely localised to the Molecular layer aspect of the Purkinje cells...Basket cell synapses?
In the lower image (higher power), the molecular layer positivity appears to be compartmentalised/restricted....
I am not very familar with AMPA GluR1 distribution in the cerebellum nor the relationships with component cells/fibres, so I ask if the positivity is not only localised to Climbing fibres but, also to Bergmann glia?
Also, there is a concentration of positivity closely localised to the Molecular layer aspect of the Purkinje cells...Basket cell synapses?
Abcam response
Many thanks for your feedback on rat cerebellum in IHC with ab31232. We invite the research community to respond to the queries you have raised on the pattern of immunoreactivity of this antibody in this tissue.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Carl Hobbs
Verified customer
提交于 Feb 01 2010