Application
Western blot
Sample
Human Cell lysate - whole cell (HT1080+MCF7)
Loading amount
10 µg
Specification
HT1080+MCF7
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Other product details
Dilution
1/1000
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 24°C · Diluent: 5%BSA in PBS+0.5% Tween 20
Secondary antibody
Name
Non-Abcam antibody was used: anti rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL plus
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 75 kDa
Additional data
Additional Notes
Observed bands from these 2 cells lines are bigger than those described by other cell line.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Oct 26 2011