概述

  • 产品名称
    Anti-GSC抗体[EPR4847]
    参阅全部 GSC 一抗
  • 描述
    兔单克隆抗体[EPR4847] to GSC
  • 宿主
    Rabbit
  • 特异性
    Negative WB result with mouse brain, heart, kidney and spleen tissue lysates, but there is a feedback this antibody detected mouse GSC with NMuMG (mouse mammary gland, normal epithelial) cell lysate. Other samples has not been tested.
  • 经测试应用
    适用于: WB, IPmore details
    不适用于: Flow Cyt,ICC or IHC-P
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide within Human GSC. The exact sequence is proprietary.

  • 阳性对照
    • Human brain, HT29, MCF7, and HepG2 lysates.
  • 常规说明

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab109024 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
IP 1/10 - 1/100.
  • 应用说明
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • 靶标

    • 功能
      Regulates chordin (CHRD). May play a role in spatial programing within discrete embryonic fields or lineage compartments during organogenesis. In concert with NKX3-2, plays a role in defining the structural components of the middle ear; required for the development of the entire tympanic ring.
    • 序列相似性
      Belongs to the paired homeobox family. Bicoid subfamily.
      Contains 1 homeobox DNA-binding domain.
    • 细胞定位
      Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Goosecoid antibody
      • goosecoid homeobox antibody
      • GSC antibody
      • GSC_HUMAN antibody
      • Homeobox protein goosecoid antibody
      see all

    图片

    • All lanes : Anti-GSC antibody [EPR4847] (ab109024) at 1/1000 dilution

      Lane 1 : Human brain lysate
      Lane 2 : HT29 lysate
      Lane 3 : MCF7 lysate
      Lane 4 : HepG2 lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 28 kDa

    文献

    ab109024 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    1-2 of 2 Abreviews or Q&A

    Application
    IHC - Wholemount
    Sample
    Mouse Tissue (Wholemount immunostaining of E7.5 mouse embryo.)
    Specification
    Wholemount immunostaining of E7.5 mouse embryo.

    Dr. Sophie Morgani

    Verified customer

    提交于 Aug 03 2018

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Mouse embryonic stem cells)
    Gel Running Conditions
    Reduced Denaturing (Bis-Tris from Invitrogen 4-12%)
    Loading amount
    18 µg
    Specification
    Mouse embryonic stem cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Dr. Maud de Dieuleveult

    Verified customer

    提交于 Jul 04 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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