Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain)
Specification
Brain
Other product details
Dilution
1/100
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: PBST
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit Alexa Fluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/1000
Additional data
Additional Notes
The antibody produced some staining in cells at the border of the fourth ventricule and a cytoplasmic staining in cells in the suprachiasmatic hypothalamic nucleus. Nothing else was stained. The staining is consistent with the expected staining in the suprachiasmatic nucleus (according to 18289018, 20211708). However, the staining is weak and not contrasted. This why I rate it as 'average'.
The pictures loaded show the staining at the level of the border of the ventricule (left) and in the suprachiasmatic nucleus, taken using the objective X20 (middle panel) and X40 (right panel). This last picture shows that the staining is located in the neuronal membrane and cytoplasm.
The sections used came from animals perfused fixed with Paraformaldehyde 4%, in phosphate buffer 0.2M. Following postfixation in the same fixative overnight, the brains were cryoprotected in sucrose 30% overnight. Brains were then cut using a cryostat and the immunostainings were performed using the ‘free floating’ technique.
The pictures loaded show the staining at the level of the border of the ventricule (left) and in the suprachiasmatic nucleus, taken using the objective X20 (middle panel) and X40 (right panel). This last picture shows that the staining is located in the neuronal membrane and cytoplasm.
The sections used came from animals perfused fixed with Paraformaldehyde 4%, in phosphate buffer 0.2M. Following postfixation in the same fixative overnight, the brains were cryoprotected in sucrose 30% overnight. Brains were then cut using a cryostat and the immunostainings were performed using the ‘free floating’ technique.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Sophie Pezet
Verified customer
提交于 Nov 22 2010