Application
Western blot
Loading amount
1e+006 cells
Gel Running Conditions
Reduced Denaturing (10% gel)
Sample
Mouse Cell lysate - whole cell (mouse embryonic stem cell)
Specification
mouse embryonic stem cell
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk (blocking buffer)
Dilution
1/500
Detection
Detection method
ECL
Bands
Specific: - kDa Non-specific: 26 kDa
Exposure
10 minute(s) and 0 second(s)
Positive control
Mouse embryonic stem cells in lanes 1 and 2.
Additional data
Additional Notes
I also used a supersensitive method of detection on the membrane, but this just brought up multiple (20+) non-specific bands in both lanes. ECL method shown. I also repeated this WB using 5% BSA as a block, but still saw no bands.
Abcam response
Thank you for sharing your data with us. ab125583 has previously been tested on mouse A20 and NIH 3T3 cells by western blot (please see datasheet). However, this abreview shows that ab125583 does not work in western blot on mouse ES cell lysates, under these conditions. Abcam would welcome feedback from other customers using this antibody.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Jan 21 2014