Application
Western blot
Sample
Human Cell lysate - other (Colorectal Cancer Cell Lines and Exosomes)
Gel Running Conditions
Reduced Denaturing (4- 20%)
Loading amount
40 µg
Specification
Colorectal Cancer Cell Lines and Exosomes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk/TBST
Dilution
1/1000
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: Anti-Rabbit IgG HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Negative control
HCT116 cells, HCT116 exosomes, HepG2 cells
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 49 kDa
Positive control
LIM1215 cells, LIM1215 exosomes
Additional data
Additional Notes
Clear bands for A33 were visible after 60 seconds of exposure (ECL detection system) in LIM1215 exosomes and LIM1215 cells at size of 49 kDa.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Fabia Fricke
Verified customer
提交于 Dec 19 2017