Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HEK 293 cells transfected with Rab27a-GFP)
Total protein in input
25 µg
Specification
HEK 293 cells transfected with Rab27a-GFP
Immuno-precipitation step
Protein G
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1%TX-100, 10% glycerol, 1XPBS
Western Blot antibody
Name
Non-Abcam antibody was used: anti mouse HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Additional data
Additional Notes
L: Lysate of HEK 293 cells expressing Rab27a-GFP
IP: Immunoprecipitation with anti GFP Ab
IP: Immunoprecipitation with anti GFP Ab
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
HERR DR. Vladimir Milenkovic
Verified customer
提交于 Jul 22 2010