Application
Western blot
Sample
Caenorhabditis elegans Cell lysate - whole cell (whole lysates)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
50 µg
Specification
whole lysates
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
8 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/250
Secondary antibody
Name
Non-Abcam antibody was used: Anti-mouse IgG, HRP-linked Antibody
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Clarity™ ECL
Exposure
30 second(s)
Bands
Specific: 37 kDa
Positive control
MOUSE LIVER SAMPLES
Additional data
Additional Notes
the antibody is specific,
but need high protein conc. samples, 50ug
and high antibody dilution (1 to 250 dilution)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Joey Wu
Verified customer
提交于 Jan 25 2017