Application
Western blot
Sample
Human Cell lysate - whole cell (bladder)
Gel Running Conditions
Reduced Denaturing (gel 10%)
Loading amount
30 µg
Treatment
lentivirus for gene knock-out
Specification
bladder
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 37 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Jinwoo Ahn
Verified customer
提交于 May 20 2016